RNA複製酶 結構 參考 导航菜单3PHU2.7.7.489026-28-2 IntEnz浏览BRENDA入口NiceZyme浏览KEGG入口代谢路径概述RCSB PDBPDBjPDBePDBsumAmiGO EGO 相关文献相关文献PF00680CL0027IPR0012052jlgstructuresRCSB PDBPDBePDBjstructure summaryPF00972IPR000208structuresRCSB PDBPDBePDBjstructure summaryMolecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domainsPMC 3038727PMID 21266548doi:10.1073/pnas.1015287108PMID 2759231doi:10.1016/0014-5793(89)80886-5A reevaluation of the higher taxonomy of viruses based on RNA polymerasesPMC 190630PMID 8709232Assembly, purification, and pre-steady-state kinetic analysis of active RNA-dependent RNA polymerase elongation complexPMC 3323022PMID 22303022doi:10.1074/jbc.M111.325530PMID 11531403doi:10.1006/viro.2001.1039PMID 9309225doi:10.1016/S0969-2126(97)00261-XPMID 10827187doi:10.1074/jbc.M002671200PMID 9878607doi:10.1006/viro.1998.9463编

己糖-葡萄糖-Glucokinase果糖-Fructokinase肝脏型Hepatic fructokinase半乳糖-磷酸果糖-1肝脏型肌肉型血小板型2核黄素莽草酸Shikimate kinase胸苷ADP-胸苷NAD+甘油泛酸甲羟戊酸丙酮酸脱氧胞苷PFP二酰甘油磷酸肌醇3Ⅰ类PI 3Ⅱ类PI 3鞘氨醇葡萄糖-1,6-二磷酸合酶磷酸甘油酸Phosphoglycerate kinase天冬氨酸Aspartate kinase肌酸磷酸甲羟戊酸Phosphomevalonate kinase腺苷酸Adenylate kinase核苷二磷酸Nucleoside-diphosphate kinase尿苷酸UMP kinase鸟苷酸Guanylate kinase硫胺素二磷酸Thiamine-diphosphate kinaseRNA酶PHPNPasePolynucleotide phosphorylase葡萄糖-1-磷酸尿苷酰基转移酶半乳糖-1-磷酸尿苷酰基转移酶mRNA加帽酶重组酶整合酶转座酶


酶RNA病毒生物化学分子生物学


酶轉錄DNARNA聚合酶義RNA病毒β-折疊α-螺旋殘基212345672.7.1011-1289312343.4.212223245678910111213





Confusion grey.svg  提示：本条目的主题不是RNA聚合酶。





















RNA複製酶
RNA-dependent RNA polymerase

RNA Replicase structure.png
RNA複製酶的結構PDB 3PHU.[1]





识别码

EC编号

2.7.7.48

CAS号

9026-28-2
数据库

IntEnz

IntEnz浏览

BRENDA（英语：BRENDA）

BRENDA入口

ExPASy（英语：ExPASy）

NiceZyme浏览

KEGG

KEGG入口

MetaCyc（英语：MetaCyc）

代谢路径

PRIAM（英语：PRIAM_enzyme-specific_profiles）

概述

PDB

RCSB PDB PDBj PDBe PDBsum

基因本体

AmiGO / EGO















RNA dependent RNA polymerase
鉴定
标志
RdRP_1

Pfam（蛋白家族查询站）

PF00680

Pfam宗系

CL0027

InterPro（蛋白数据整合站）

IPR001205

SCOP（蛋白结构分类数据站）

2jlg













RNA-directed RNA polymerase, flaviviral
鉴定
标志
RNA_pol_flaviviral

Pfam（蛋白家族查询站）

PF00972

InterPro（蛋白数据整合站）

IPR000208







RNA複製酶（RNA replicase），或名RNA依賴性RNA聚合酶（RNA-dependent RNA polymerase 縮寫RdRp或RDR），係一類能以複製RNA的酶。值得注意的是，這類酶和在轉錄中扮演重要角色、以DNA爲模板合成RNA的RNA聚合酶不是同一類酶。


RNA複製酶對於那些反義RNA病毒來說，是一種至關重要的蛋白質[2][3]。RNA複製酶能合成一條RNA單鏈的互補鏈[4][5]。



結構


所有的RNA複製酶和不少RNA聚合酶都有一個摺疊結構。這個摺疊結構與三個亞結構域相連。上面三個結構域的形狀和人的右手想像，因而分別被稱爲「四指」（fingers）、「手掌」（palm）、「拇指」（thumb）[6]。其中，只有手掌亞結構域有一個由四條反向平行的多肽鏈構成的β-折疊結構和兩個α-螺旋結構，在各類RNA複製酶中高度保守。其中，手掌亞結構域有三個高度保守的基元（A、B，以及C）。基元A（D-x(4,5)-D）和基元C（GDD）在空間上並列。這兩個基元中的天冬氨酸殘基與鎂離子和錳離子相連。而B基元中的天冬酰胺殘基則與在脫氧三磷酸核苷中選擇三磷酸核糖核苷的過程有關，上述過程可以確保合成的是RNA而不是DNA[7]。RNA複製酶的結構域的組織形式保守[8]，其催化中心也是一樣。RNA複製酶的催化中心是由一系列包含保守的氨基酸殘基的基元組成的，即使在同源性不高的編碼序列下也保守。



參考




  1. ^ Akutsu, M; Ye, Y; Virdee, S; Chin, JW; Komander, D. Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains. Proc. Natl. Acad. Sci. U.S.A. February 2011, 108 (6): 2228–33. PMC 3038727. PMID 21266548. doi:10.1073/pnas.1015287108. 


  2. ^ Koonin EV, Gorbalenya AE, Chumakov KM. Tentative identification of RNA-dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases. FEBS Lett. July 1989, 252 (1–2): 42–6. PMID 2759231. doi:10.1016/0014-5793(89)80886-5. 


  3. ^ Zanotto PM, Gibbs MJ, Gould EA, Holmes EC. A reevaluation of the higher taxonomy of viruses based on RNA polymerases. J. Virol. September 1996, 70 (9): 6083–96. PMC 190630. PMID 8709232. 


  4. ^ Jin, Z; Leveque, V; Ma, H; Johnson, K. A.; Klumpp, K. Assembly, purification, and pre-steady-state kinetic analysis of active RNA-dependent RNA polymerase elongation complex. Journal of Biological Chemistry. 2012, 287 (13): 10674–83. PMC 3323022. PMID 22303022. doi:10.1074/jbc.M111.325530. 


  5. ^ Kao CC, Singh P, Ecker DJ. De novo initiation of viral RNA-dependent RNA synthesis. Virology. September 2001, 287 (2): 251–60. PMID 11531403. doi:10.1006/viro.2001.1039. 


  6. ^ Hansen JL, Long AM, Schultz SC. Structure of the RNA-dependent RNA polymerase of poliovirus. Structure. August 1997, 5 (8): 1109–22. PMID 9309225. doi:10.1016/S0969-2126(97)00261-X. 


  7. ^ Gohara DW, Crotty S, Arnold JJ, Yoder JD, Andino R, Cameron CE. Poliovirus RNA-dependent RNA polymerase (3Dpol): structural, biochemical, and biological analysis of conserved structural motifs A and B. J. Biol. Chem. August 2000, 275 (33): 25523–32. PMID 10827187. doi:10.1074/jbc.M002671200. 


  8. ^ O'Reilly EK, Kao CC. Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology. December 1998, 252 (2): 287–303. PMID 9878607. doi:10.1006/viro.1998.9463. 













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转移酶：含磷基团的激酶类（EC 2.7）

2.7.1-2.7.4：磷酸转移酶类（英语：phosphotransferase）/激酶
（PO4）










2.7.1：以羟基为受体

  • 己糖-

  • 葡萄糖-（英语：Glucokinase）


  • 果糖-（英语：Fructokinase）
    • 肝脏型（英语：Hepatic fructokinase）

  • 半乳糖-


  • 磷酸果糖-
    • 1

    • 肝脏型

    • 肌肉型

    • 血小板型

    • 2


  • 核黄素

  • 莽草酸（英语：Shikimate kinase）


  • 胸苷

    • ADP-胸苷）

  • NAD+

  • 甘油

  • 泛酸

  • 甲羟戊酸

  • 丙酮酸

  • 脱氧胞苷

  • PFP

  • 二酰甘油


  • 磷酸肌醇3
    • Ⅰ类PI 3

    • Ⅱ类PI 3


  • 鞘氨醇

  • 葡萄糖-1,6-二磷酸合酶


2.7.2（英语：List_of_EC_numbers_(EC_2)#EC_2.7.2:_Phosphotransferases_with_a_carboxy_group_as_acceptor）：以羧基为受体

  • 磷酸甘油酸（英语：Phosphoglycerate kinase）

  • 天冬氨酸（英语：Aspartate kinase）


2.7.3：以含氮基团为受体

  • 肌酸

2.7.4：以磷酸基团为受体

  • 磷酸甲羟戊酸（英语：Phosphomevalonate kinase）

  • 腺苷酸（英语：Adenylate kinase）

  • 核苷二磷酸（英语：Nucleoside-diphosphate kinase）

  • 尿苷酸（英语：UMP kinase）

  • 鸟苷酸（英语：Guanylate kinase）

  • 硫胺素二磷酸（英语：Thiamine-diphosphate kinase）



2.7.6（英语：List of EC numbers (EC 2)#EC_2.7.6:_Diphosphotransferases）：二磷酸转移酶类（英语：Diphosphotransferase）
(P2O7)

  • 核糖磷酸二磷酸激酶（英语：Ribose-phosphate diphosphokinase）

  • 硫胺素二磷酸激酶（英语：Thiamine diphosphokinase）


2.7.7：核苷酸基转移酶












聚合酶





DNA聚合酶

DNA指导的DNA聚合酶

DNA聚合酶Ⅰ

DNA聚合酶Ⅱ（英语：DNA polymerase II）

DNA聚合酶Ⅲ全酶

DNA聚合酶Ⅳ（英语：DNA polymerase IV）

DNA聚合酶Ⅴ（英语：DNA polymerase V）

RNA指导的DNA聚合酶


逆转录酶
端粒酶


DNA核苷酸基外转移酶/末端脫氧核苷酸轉移酶
RNA核苷酸转移酶


DNA指导的RNA聚合酶RNA聚合酶

RNA聚合酶Ⅰ（英语：RNA polymerase I）

RNA聚合酶Ⅱ

RNA聚合酶Ⅲ（英语：RNA polymerase III）

RNA聚合酶Ⅳ（英语：RNA polymerase IV）

RNA聚合酶V

引发酶


RNA指导的RNA聚合酶RNA複製酶


磷酸解作用
由3'端至5'端的核糖核酸外切酶

  • RNA酶PH

  • PNPase（英语：Polynucleotide phosphorylase）

尿苷酰转移酶

  • 葡萄糖-1-磷酸尿苷酰基转移酶

  • 半乳糖-1-磷酸尿苷酰基转移酶

鸟苷酰转移酶

  • mRNA加帽酶
其它


  • 重组酶（整合酶）

  • 转座酶



2.7.8：杂项





磷脂酰基转移酶类

  • CDP-甘油二酯—甘油-3-磷酸3-磷脂酰基转移酶

  • CDP-甘油二酯—丝氨酸O-磷脂酰基转移酶

  • CDP-甘油二酯—肌醇3-磷脂酰基转移酶

  • CDP-甘油二酯—胆碱O-磷脂酰基转移酶

糖基-1-磷酸转移酶类

  • N-乙酰葡糖胺-1-磷酸转移酶

2.7.10-2.7.13：蛋白激酶类
（PO4；蛋白质受体）










2.7.10：蛋白酪氨酸

  • 见酪氨酸激酶类

2.7.11：蛋白丝氨酸/苏氨酸

  • 见丝氨酸/苏氨酸特异性蛋白激酶类

2.7.12：蛋白双特异性

  • 见丝氨酸/苏氨酸特异性蛋白激酶类

2.7.13：蛋白组氨酸

  • 蛋白组氨酸前激酶

  • 蛋白组氨酸远激酶

  • 組氨酸激酶



EC 1.1/2/3/4/5/6/7/8/9/10/11/12/13/14/15/16/17/18/19/20/21/22
 ·
2.1/2/3/4/5/6/7(2.7.10/11-12)/8/9
 ·
3.1/2/3/4(3.4.21/22/23/24)/5/6/7/8/9/10/11/12/13
 ·


4.1/2/3/4/5/6 · 5.1/2/3/4/5/99 · 6.1-3（英语：Template:Ligases CO CS and CN）/4/5-6

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